Job Title: Scientist II (Flow Cytometry, Tissue Culture, Biochemistry)

Location: San Diego, CA

Duration: 12+ Months

Client: Medical Device Company

Job Category: Research & Development

Employment Type: Contract on W2 (Need US Citizens Or GC Holders Only)

No H1B’s

• Work hours: Regular hours (8-5)
• Remote or onsite: Onsite
• 3 Must haves on the resume: Flow Cytometry, Tissue Culture, Biochemistry

Scientist II-Antibody Development

Job Summary
We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Discovery team. This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to recombinant antibody expression and testing new antibodies for use in flow cytometry applications. The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology. The successful candidate is expected to set up and execute experiments, be detail oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.

Duties And Responsibilities
• Responsible for transfection and expression of antibodies in cell lines. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Assist with protein conjugation and molecular biology processes.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge And Skills
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
• Familiarity with molecular biology (DNA preparation) and protein conjugation (fluorophore, small molecule and fluorescent proteins and protein purification methods).
• Assay automation.
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Effective analytical and problem-solving skills.

Education And Experience
A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-5 years of laboratory experience required. Flow cytometry and/or tissue culture experience required.