Position : Biochemist I (Must Have Tissue culture, Cell Culture, Pipetting &Dilution)

Location : San Diego, CA

Duration : 12 Months

Total Hours/week : 40.00

1^st Shift

Client: Medical Device Company

Job Category Healthcare

Level Of Experience: Entry Level

Employment Type: Contract on W2 (Need US Citizens Or GC Holders Only)

Work hours: 8 to 5 with ½ hour lunch and 15 minutes break twice a day.

3 Must haves:

• Tissue culture sterile technique
• Hybridoma cell culture
• Centrifugation and/or Tangential Flow System experience

Job Description:

• The Biochemist I is expected to work independently, perform all laboratory functions that involved in Tissue Culture such as Hybridoma roller bottle cell culture, ascites production, insect cell shaker culture, recombinant protein production, in-process assays and safety procedures. The Biochemist I is expected to learn sterile technique and make antibodies.
• The biochemist I is expected to be capable to complete assigned tissue culture procedures with good quality by following SOPs and batch records.
• The position is expected to perform data entries timely and properly. The biochemist I is expected to learn more procedures regularly based on the production requirements.
• The position is expected to finish assignments on time to help keep up Tissue Culture productivity.
• In addition, biochemist I is responsible for completing their individual training plan based on the approved IIGs annually.
• The position works closely with senior biochemist to ensure that the assignments are done properly before due dates.
• The position will support and participate in continuous improvement projects as identified by the Executive Steering Committee and assigned by the manager.

Position Qualifications

Education:

• Typically requires BS degree at a 4 year degree program in Science or equivalent combination of related education and experience.
• Work Experience: Typically requires 0-2 years' experience in a Biotech/ Life Science manufacturing environment. Have some working knowledge in the science disciplines and operation of basic lab equipment.

Required Licenses:

• None

Techniques Required:

• Pipetting
• Dilution
• Centrifugation
• Sterile technique

Techniques Desired:

• Cell culture

Knowledge and Skills Required:

• Demonstrated ability to use laboratory instruments and applicable computer software.
• Knowledge of general laboratory safety
• Effective interpersonal skills, verbal and written communication
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, complete and legible records and logs.
• Possess analytical problem solving skills.
• Must be able to lift a minimum of 25 pounds weight.

Physical Requirements:

• General physical requirements: Sedentary work
• Visual acuity requirements: Inspection / Administrative.
• Motion and sensory requirements: Talking, hearing and walking.

Principal Accountabilities: (Prioritized Activities)

• Implement and follow all new and current department policies and procedures per departmental SOP's and Batch Records for all manufacturing functions within the department.
• Follow all departmental guidelines and procedures with minimal Departmental Supervision.
• Maintain all manufacturing batch records.
• Be able to perform data entry in all required data bases
• Other duties as assigned by Supervisor.

Responsibilities:

• 60-80% Operations / 40-20% technical training

• Responsible for learning the sterile Tissue Culture technique to independently work under Biosafety hood.
• Follow the Tissue Culture SOP to make different kinds monoclonal antibody and cell related products by Hybridoma roller bottle cell culture and ascites production and other mammalian cell related products.
• Follow the Tissue Culture SOP to perform insect cell shaker bioreactor for producing recombinant protein by using Baculovirus or E.coli expression system.
• Complete Batch Records accurately and in a timely manner.
• Follow all SOP's and Work Instructions precisely
• Perform clarification and concentration of Tissue Culture supernatant using the Tangential Flow system and /or centrifugation.
• Perform in-process assays Nephenomelter, Mycoalert and isotype ELISA.
• Help maintain the cell bank inventory by accurate retrieval and placement of vials from LN2 freezer.
• Promote a safe working environment. Follows Client Environmental, Health and Safety (EH&S) policies and procedures.
• Takes responsibility for safety in immediate work area.
• Participates in EH&S programs. Notifies supervisors of all observed hazardous conditions or unsafe work practices.
• May provide recommendations on maintaining the safety of the work environment.
• Perform other related duties and assignments as required.